Six dinucleotide repeat polymorphisms on chromosome 7

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Genetic mapping of dinucleotide repeat polymorphisms and von Hippel-Lindau disease on chromosome 3p25-26.

A genetic map of highly polymorphic microsatellite markers spanning the von Hippel-Lindau region (VHL) of 3p25 was constructed using the CEPH reference pedigrees. A greater than 1000:1 odds map of pter-D3S1038-RAF1-D3S651-D3S656-D3S110- D3S1255-cen was found. Genotyping of six multigenerational VHL families showed the region surrounding the D3S1038 marker to be the most likely location for the ...

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Dinucleotide repeat polymorphisms at the DXS453, DXS454 and DXS458 loci

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Dinucleotide repeat polymorphisms at the D17S250 and D17S261 loci.

Other Comments: Conditions for the amplification reactions were as described in the reference except that samples were processed through 27 temperature cycles consisting of 1 min at 94°, 2 min at 55° and 2 min at 72°. Sizes of the alleles were determined by comparison to mp8 DNA sequencing ladders. The most intense band for each allele on the denaturing polyacrylamide gels was use to obtain all...

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Dinucleotide repeat polymorphisms at the D8S85, D8S87, and D8S88 loci

Frequency: Mfdl8: Estimated from 116 chromosomes of unrelated CEPH family grandparents (Caucasians). PIC = 0.69. Allele (bp) Frequency Allele (bp) Frequency 84 0.01 76 0.22 82 0.36 74 0.22 80 0.19 Mfd39: Estimated from 96 chromosomes of unrelated CEPH family grandparents (Caucasians). PIC = 0.65. Allele (bp) Frequency Allele (bp) Frequency 157 0.02 149 0.14 155 0.18 147 0.04 153 0.06 145 0.05 1...

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Dinucleotide repeat polymorphism in a gene on chromosome 20 encoding a G-protein coupled receptor (D20S32e).

MendeUan Inheritance: Co-dominant inheritance was observed in a five-generation pedigree in which 113 individuals were typed. Other Comments: The PCR was performed using P-labeled GPR 112-1 and unlabeled GPR 112-2 for 30 cycles: denaturation at 94°C for 1 min; annealing for 55°C for 1.5 min; and extension at 72°C for 3 min. The PCR products were analyzed on a 5% denaturing polyacrylamide gel (F...

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ژورنال

عنوان ژورنال: Japanese Journal of Human Genetics

سال: 1994

ISSN: 0021-5074

DOI: 10.1007/bf01892393